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Assay Detail

CHEMBL902716

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human DPP4 in Caco-2 cells by fluorescene assay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Caco-2
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Dipeptidyl peptidase 4 (CHEMBL284)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

8-(3-(R)-aminopiperidin-1-yl)-7-but-2-ynyl-3-methyl-1-(4-methyl-quinazolin-2-ylmethyl)-3,7-dihydropurine-2,6-dione (BI 1356), a highly potent, selective, long-acting, and orally bioavailable DPP-4 inhibitor for the treatment of type 2 diabetes.
Eckhardt M, Langkopf E, Mark M, Tadayyon M, Thomas L, Nar H, Pfrengle W, Guth B, Lotz R, Sieger P, Fuchs H, Himmelsbach F.
J Med Chem (2007) 50 : 6450 -6453
PubMed 18052023 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.63 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL237289 1 9.00
CHEMBL237500 LINAGLIPTIN 4.0 1 9.00
CHEMBL391089 1 8.70
CHEMBL237067 1 8.52
CHEMBL237499 1 8.52
CHEMBL237287 1 8.40
CHEMBL429450 1 8.40
CHEMBL365000 1 8.30
CHEMBL237488 1 8.22
CHEMBL391307 1 8.10
CHEMBL237288 1 8.05
CHEMBL237279 1 7.46
CHEMBL237281 1 7.25
CHEMBL399216 1 7.09
CHEMBL237280 1 7.06
CHEMBL235792 1 6.70
CHEMBL391634 1 6.55
CHEMBL235352 1 6.24
CHEMBL235134 1 5.55
CHEMBL235133 1 5.41

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL237289 IC50 = 1.0 nM 9.00
CHEMBL237500 LINAGLIPTIN IC50 = 1.0 nM 9.00
CHEMBL391089 IC50 = 2.0 nM 8.70
CHEMBL237067 IC50 = 3.0 nM 8.52
CHEMBL237499 IC50 = 3.0 nM 8.52
CHEMBL237287 IC50 = 4.0 nM 8.40
CHEMBL429450 IC50 = 4.0 nM 8.40
CHEMBL365000 IC50 = 5.0 nM 8.30
CHEMBL237488 IC50 = 6.0 nM 8.22
CHEMBL391307 IC50 = 8.0 nM 8.10
CHEMBL237288 IC50 = 9.0 nM 8.05
CHEMBL237279 IC50 = 35.0 nM 7.46
CHEMBL237281 IC50 = 56.0 nM 7.25
CHEMBL399216 IC50 = 82.0 nM 7.09
CHEMBL237280 IC50 = 88.0 nM 7.06
CHEMBL235792 IC50 = 200.0 nM 6.70
CHEMBL391634 IC50 = 284.0 nM 6.55
CHEMBL235352 IC50 = 580.0 nM 6.24
CHEMBL235134 IC50 = 2800.0 nM 5.55
CHEMBL235133 IC50 = 3900.0 nM 5.41