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Assay Detail

CHEMBL903949

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat recombinant nNOS expressed in Escherichia coli assessed as nitric oxide production by hemoglobin capture assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Nitric oxide synthase 1 (CHEMBL3048)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Selective L-nitroargininylaminopyrrolidine and L-nitroargininylaminopiperidine neuronal nitric oxide synthase inhibitors.
Seo J, Martásek P, Roman LJ, Silverman RB.
Bioorg Med Chem (2007) 15 : 1928 -1938
PubMed 17239601 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 6.01 6.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL226390 1 6.66
CHEMBL228077 1 6.48
CHEMBL226447 1 6.33
CHEMBL375943 1 6.32
CHEMBL375895 1 6.21
CHEMBL376145 1 5.97
CHEMBL226448 1 5.96
CHEMBL226445 1 5.75
CHEMBL226499 1 5.65
CHEMBL375056 1 5.62
CHEMBL226446 1 5.12

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL226390 Ki = 220.0 nM 6.66
CHEMBL228077 Ki = 330.0 nM 6.48
CHEMBL226447 Ki = 470.0 nM 6.33
CHEMBL375943 Ki = 480.0 nM 6.32
CHEMBL375895 Ki = 610.0 nM 6.21
CHEMBL376145 Ki = 1080.0 nM 5.97
CHEMBL226448 Ki = 1090.0 nM 5.96
CHEMBL226445 Ki = 1760.0 nM 5.75
CHEMBL226499 Ki = 2260.0 nM 5.65
CHEMBL375056 Ki = 2380.0 nM 5.62
CHEMBL226446 Ki = 7620.0 nM 5.12