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Assay Detail

CHEMBL907229

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [His5, D-Tyr6]GnRH from GnRHR F309L mutant expressed in COS-7 cells
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS-7
Confidence 9 — Direct single protein target
Curated By Expert

Target

Gonadotropin-releasing hormone receptor (CHEMBL1855)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Determination of the binding mode of thienopyrimidinedione antagonists to the human gonadotropin releasing hormone receptor using structure-activity relationships, site-directed mutagenesis, and homology modeling.
Betz SF, Lio FM, Gao Y, Reinhart GJ, Guo Z, Mesleh MF, Zhu YF, Struthers RS.
J Med Chem (2006) 49 : 6170 -6176
PubMed 17034124 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.58 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL22055 SUFUGOLIX 2.0 1 8.22
CHEMBL435167 1 8.05
CHEMBL280212 1 7.00
CHEMBL385175 1 6.05
CHEMBL332138 1 6.02
CHEMBL332355 1 5.96
CHEMBL387006 1 5.75
CHEMBL333518 1 5.58
CHEMBL262557 1 -
CHEMBL120260 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL22055 SUFUGOLIX IC50 = 6.0 nM 8.22
CHEMBL435167 IC50 = 9.0 nM 8.05
CHEMBL280212 IC50 = 100.0 nM 7.00
CHEMBL385175 IC50 = 900.0 nM 6.05
CHEMBL332138 IC50 = 950.0 nM 6.02
CHEMBL332355 IC50 = 1100.0 nM 5.96
CHEMBL387006 IC50 = 1800.0 nM 5.75
CHEMBL333518 IC50 = 2650.0 nM 5.58
CHEMBL262557 IC50 > 10000.0 nM -
CHEMBL120260 IC50 > 10000.0 nM -