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Assay Detail

CHEMBL908432

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]Nicotine from alpha4beta2 nAChR in rat cortical membranes
6
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Subcellular Membrane
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Epibatidine isomers and analogues: structure-activity relationships.
White R, Malpass JR, Handa S, Richard Baker S, Broad LM, Folly L, Mogg A.
Bioorg Med Chem Lett (2006) 16 : 5493 -5497
PubMed 16934977 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 9.84 10.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL298517 (+)-EPIBATIDINE 1 10.72
CHEMBL298826 (-)-EPIBATIDINE 1 10.70
CHEMBL185667 2 10.35
CHEMBL535619 1 10.25
CHEMBL535389 (+/-)-HOMOEPIBATIDINE 1 9.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL298517 (+)-EPIBATIDINE Ki = 0.019 nM 10.72
CHEMBL298826 (-)-EPIBATIDINE Ki = 0.02 nM 10.70
CHEMBL185667 Ki = 0.045 nM 10.35
CHEMBL535619 Ki = 0.056 nM 10.25
CHEMBL535389 (+/-)-HOMOEPIBATIDINE Ki = 0.23 nM 9.64
CHEMBL185667 Ki = 40.0 nM 7.40