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Assay Detail

CHEMBL909736

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]MLA binding to alpha-7 nAChR in rat brain membrane
9
Total Activities
9
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Expert

Target

Neuronal acetylcholine receptor subunit alpha-7 (CHEMBL4980)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Des-keto lobeline analogs with increased potency and selectivity at dopamine and serotonin transporters.
Zheng G, Horton DB, Deaciuc AG, Dwoskin LP, Crooks PA.
Bioorg Med Chem Lett (2006) 16 : 5018 -5021
PubMed 16905316 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 - -
Ki 4 5.33 5.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL125511 8R-hydroxylobelane 1 5.92
CHEMBL425617 1 5.77
CHEMBL122270 LOBELINE 2.0 1 5.20
CHEMBL122317 1 4.41
CHEMBL213613 1 -
CHEMBL213148 1 -
CHEMBL377539 1 -
CHEMBL217815 1 -
CHEMBL124219 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL125511 8R-hydroxylobelane Ki = 1210.0 nM 5.92
CHEMBL425617 Ki = 1700.0 nM 5.77
CHEMBL122270 LOBELINE Ki = 6260.0 nM 5.20
CHEMBL122317 Ki = 39300.0 nM 4.41
CHEMBL213613 IC50 > 100000.0 nM -
CHEMBL213148 IC50 > 100000.0 nM -
CHEMBL377539 IC50 > 100000.0 nM -
CHEMBL217815 IC50 > 100000.0 nM -
CHEMBL124219 IC50 > 100000.0 nM -