Assay Detail
Binding
CHEMBL914522
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Toxoplasma gondii DHFR
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Toxoplasma gondii |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Target
Bifunctional dihydrofolate reductase-thymidylate synthase (CHEMBL2425) ↗| Type | SINGLE PROTEIN |
| Organism | Toxoplasma gondii |
Publication
Synthesis of N-{4-[(2,4-diamino-5-methyl-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)thio]benzoyl}-L-glutamic acid and N-{4-[(2-amino-4-oxo-5-methyl-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-6-yl)thio]benzoyl}-L-glutamic acid as dual inhibitors of dihydrofolate reductase and thymidylate synthase and as potential antitumor agents.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 6.51 | 7.96 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | 7.96 |
| CHEMBL389643 | — | — | 1 | 6.77 |
| CHEMBL422395 | — | — | 1 | 6.66 |
| CHEMBL225072 | PEMETREXED | 4.0 | 1 | 6.34 |
| CHEMBL224974 | — | — | 1 | 5.66 |
| CHEMBL225071 | RALTITREXED | 4.0 | 1 | 5.64 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL34259 | METHOTREXATE | IC50 | = | 11.0 | nM | 7.96 |
| CHEMBL389643 | — | IC50 | = | 170.0 | nM | 6.77 |
| CHEMBL422395 | — | IC50 | = | 220.0 | nM | 6.66 |
| CHEMBL225072 | PEMETREXED | IC50 | = | 460.0 | nM | 6.34 |
| CHEMBL224974 | — | IC50 | = | 2200.0 | nM | 5.66 |
| CHEMBL225071 | RALTITREXED | IC50 | = | 2300.0 | nM | 5.64 |