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Assay Detail

CHEMBL915319

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at mouse MC1R expressed in HEK293 cells by beta-galactosidase assay
11
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Melanocyte-stimulating hormone receptor (CHEMBL4077)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Stereochemical studies of the monocyclic agouti-related protein (103-122) Arg-Phe-Phe residues: conversion of a melanocortin-4 receptor antagonist into an agonist and results in the discovery of a potent and selective melanocortin-1 agonist.
Joseph CG, Wang XS, Scott JW, Bauzo RM, Xiang Z, Richards NG, Haskell-Luevano C.
J Med Chem (2004) 47 : 6702 -6710
PubMed 15615519 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 11 7.25 10.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL430239 MELATONAN 1 10.68
CHEMBL441738 AFAMELANOTIDE 4.0 1 10.64
CHEMBL214332 INTERMEDINE 2.0 1 9.55
CHEMBL2448525 1 7.19
CHEMBL2304247 1 7.03
CHEMBL3350327 2 6.92
CHEMBL2304248 1 5.76
CHEMBL2369131 1 5.43
CHEMBL2304246 1 5.28
CHEMBL2304250 1 5.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL430239 MELATONAN EC50 = 0.021 nM 10.68
CHEMBL441738 AFAMELANOTIDE EC50 = 0.023 nM 10.64
CHEMBL214332 INTERMEDINE EC50 = 0.28 nM 9.55
CHEMBL2448525 EC50 = 64.0 nM 7.19
CHEMBL2304247 EC50 = 93.0 nM 7.03
CHEMBL3350327 EC50 = 120.0 nM 6.92
CHEMBL3350327 EC50 = 960.0 nM 6.02
CHEMBL2304248 EC50 = 1750.0 nM 5.76
CHEMBL2369131 EC50 = 3750.0 nM 5.43
CHEMBL2304246 EC50 = 5270.0 nM 5.28
CHEMBL2304250 EC50 = 6330.0 nM 5.20