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Assay Detail

CHEMBL915320

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at mouse MC3R expressed in HEK293 cells by beta-galactosidase assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Expert

Target

Melanocortin receptor 3 (CHEMBL4774)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Stereochemical studies of the monocyclic agouti-related protein (103-122) Arg-Phe-Phe residues: conversion of a melanocortin-4 receptor antagonist into an agonist and results in the discovery of a potent and selective melanocortin-1 agonist.
Joseph CG, Wang XS, Scott JW, Bauzo RM, Xiang Z, Richards NG, Haskell-Luevano C.
J Med Chem (2004) 47 : 6702 -6710
PubMed 15615519 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 7 6.87 10.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL441738 AFAMELANOTIDE 4.0 1 10.07
CHEMBL430239 MELATONAN 1 9.77
CHEMBL214332 INTERMEDINE 2.0 1 9.28
CHEMBL2304248 1 5.26
CHEMBL3350327 1 4.86
CHEMBL2369131 1 4.65
CHEMBL2448525 1 4.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL441738 AFAMELANOTIDE EC50 = 0.085 nM 10.07
CHEMBL430239 MELATONAN EC50 = 0.17 nM 9.77
CHEMBL214332 INTERMEDINE EC50 = 0.52 nM 9.28
CHEMBL2304248 EC50 = 5510.0 nM 5.26
CHEMBL3350327 EC50 = 13700.0 nM 4.86
CHEMBL2369131 EC50 = 22600.0 nM 4.65
CHEMBL2448525 EC50 = 63300.0 nM 4.20