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Assay Detail

CHEMBL916827

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Growth inhibition of MTX-resistant human CCRF-CEM R2 cells
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type R2
Confidence 1 — Organism
Curated By Autocuration

Target

R2 (CHEMBL614385)
Type CELL-LINE
Organism Homo sapiens

Publication

Benzoyl ring halogenated classical 2-amino-6-methyl-3,4-dihydro-4-oxo-5-substituted thiobenzoyl-7H-pyrrolo[2,3-d]pyrimidine antifolates as inhibitors of thymidylate synthase and as antitumor agents.
Gangjee A, Jain HD, McGuire JJ, Kisliuk RL.
J Med Chem (2004) 47 : 6730 -6739
PubMed 15615522 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 5.40 5.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL34259 METHOTREXATE 4.0 1 5.68
CHEMBL388340 1 5.61
CHEMBL224868 1 4.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL34259 METHOTREXATE EC50 = 2100.0 nM 5.68
CHEMBL388340 EC50 = 2450.0 nM 5.61
CHEMBL224868 EC50 = 12000.0 nM 4.92