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Assay Detail

CHEMBL919087

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]Ro 15-1788 from human recombinant GABAA alpha-1-beta-3-gamma-2 receptor expressed in L(tk-) cells
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

GABA-A receptor; alpha-1/beta-3/gamma-2 (CHEMBL2094121)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

7-(1,1-Dimethylethyl)-6-(2-ethyl-2H-1,2,4-triazol-3-ylmethoxy)-3-(2-fluorophenyl)-1,2,4-triazolo[4,3-b]pyridazine: a functionally selective gamma-aminobutyric acid(A) (GABA(A)) alpha2/alpha3-subtype selective agonist that exhibits potent anxiolytic activity but is not sedating in animal models.
Carling RW, Madin A, Guiblin A, Russell MG, Moore KW, Mitchinson A, Sohal B, Pike A, Cook SM, Ragan IC, McKernan RM, Quirk K, Ferris P, Marshall G, Thompson SA, Wafford KA, Dawson GR, Atack JR, Harrison T, Castro JL, Street LJ.
J Med Chem (2005) 48 : 7089 -7092
PubMed 16279764 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 8.69 9.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL375742 1 9.64
CHEMBL200177 MK-0777 2.0 1 9.57
CHEMBL225631 1 9.44
CHEMBL224561 1 9.42
CHEMBL224175 1 9.11
CHEMBL224134 1 8.96
CHEMBL224608 1 8.21
CHEMBL12 DIAZEPAM 4.0 1 7.85
CHEMBL911 ZOLPIDEM 4.0 1 7.57
CHEMBL178942 1 7.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL375742 Ki = 0.23 nM 9.64
CHEMBL200177 MK-0777 Ki = 0.27 nM 9.57
CHEMBL225631 Ki = 0.36 nM 9.44
CHEMBL224561 Ki = 0.38 nM 9.42
CHEMBL224175 Ki = 0.78 nM 9.11
CHEMBL224134 Ki = 1.1 nM 8.96
CHEMBL224608 Ki = 6.2 nM 8.21
CHEMBL12 DIAZEPAM Ki = 14.0 nM 7.85
CHEMBL911 ZOLPIDEM Ki = 27.0 nM 7.57
CHEMBL178942 Ki = 71.0 nM 7.15