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Assay Detail

CHEMBL921229

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human Pgp mediated [3H]vinblastine transport in human Caco-2 cells
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Caco-2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Small P-gp modulating molecules: SAR studies on tetrahydroisoquinoline derivatives.
Colabufo NA, Berardi F, Cantore M, Perrone MG, Contino M, Inglese C, Niso M, Perrone R, Azzariti A, Simone GM, Porcelli L, Paradiso A.
Bioorg Med Chem (2008) 16 : 362 -373
PubMed 17936633 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 4.98 6.26

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL53325 1 6.26
CHEMBL260599 1 5.79
CHEMBL396298 ELACRIDAR 2.0 1 5.70
CHEMBL405448 1 5.31
CHEMBL259582 1 5.20
CHEMBL259367 1 5.09
CHEMBL264424 1 5.09
CHEMBL405372 1 4.99
CHEMBL408993 1 4.82
CHEMBL415560 1 4.82
CHEMBL259366 1 4.75
CHEMBL6966 VERAPAMIL 4.0 1 4.70
CHEMBL261802 1 4.50
CHEMBL260499 1 4.45
CHEMBL160 CYCLOSPORINE 4.0 1 4.10
CHEMBL260377 1 4.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL53325 EC50 = 550.0 nM 6.26
CHEMBL260599 EC50 = 1640.0 nM 5.79
CHEMBL396298 ELACRIDAR EC50 = 2000.0 nM 5.70
CHEMBL405448 EC50 = 4860.0 nM 5.31
CHEMBL259582 EC50 = 6350.0 nM 5.20
CHEMBL259367 EC50 = 8150.0 nM 5.09
CHEMBL264424 EC50 = 8200.0 nM 5.09
CHEMBL405372 EC50 = 10200.0 nM 4.99
CHEMBL408993 EC50 = 15200.0 nM 4.82
CHEMBL415560 EC50 = 15000.0 nM 4.82
CHEMBL259366 EC50 = 17900.0 nM 4.75
CHEMBL6966 VERAPAMIL EC50 = 20000.0 nM 4.70
CHEMBL261802 EC50 = 31600.0 nM 4.50
CHEMBL260499 EC50 = 35500.0 nM 4.45
CHEMBL160 CYCLOSPORINE EC50 = 80000.0 nM 4.10
CHEMBL260377 EC50 = 83700.0 nM 4.08