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Assay Detail

CHEMBL924308

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of hypoxia induced HIF1-alpha transcriptional activity in human AGS cells by reporter gene assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type AGS
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hypoxia-inducible factor 1-alpha (CHEMBL4261)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of (aryloxyacetylamino)-isonicotinic/nicotinic acid analogues as potent hypoxia-inducible factor (HIF)-1alpha inhibitors.
Boovanahalli SK, Jin X, Jin Y, Kim JH, Dat NT, Hong YS, Lee JH, Jung SH, Lee K, Lee JJ.
Bioorg Med Chem Lett (2007) 17 : 6305 -6310
PubMed 17884495 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.58 6.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL219673 1 6.46
CHEMBL373396 1 6.16
CHEMBL430393 1 6.10
CHEMBL401390 1 6.05
CHEMBL248836 1 5.89
CHEMBL249027 1 5.75
CHEMBL333985 1 5.70
CHEMBL399636 1 5.70
CHEMBL249028 1 5.30
CHEMBL249023 1 5.28
CHEMBL403789 1 4.96
CHEMBL248835 1 4.73
CHEMBL248650 1 4.52
CHEMBL250671 1 -
CHEMBL399845 1 -
CHEMBL398897 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL219673 IC50 = 350.0 nM 6.46
CHEMBL373396 IC50 = 700.0 nM 6.16
CHEMBL430393 IC50 = 800.0 nM 6.10
CHEMBL401390 IC50 = 900.0 nM 6.05
CHEMBL248836 IC50 = 1300.0 nM 5.89
CHEMBL249027 IC50 = 1800.0 nM 5.75
CHEMBL333985 IC50 = 2000.0 nM 5.70
CHEMBL399636 IC50 = 2000.0 nM 5.70
CHEMBL249028 IC50 = 5000.0 nM 5.30
CHEMBL249023 IC50 = 5200.0 nM 5.28
CHEMBL403789 IC50 = 11000.0 nM 4.96
CHEMBL248835 IC50 = 18600.0 nM 4.73
CHEMBL248650 IC50 = 30000.0 nM 4.52
CHEMBL250671 IC50 > 10000.0 nM -
CHEMBL399845 IC50 > 30000.0 nM -
CHEMBL398897 IC50 > 30000.0 nM -