Assay Detail
Binding
CHEMBL929247
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of fluorescent 5-(3-(3-(4-((4-((7-(hydroxyamino)-7-oxoheptyl)carbamoyl)phenylamino)methyl)-1H-1,2,3-triazol-1-yl)propyl)thioureido)-2-(3-hydroxy-6-oxo-6H-xanthen-9-yl)benzoic acid from HDAC in human HeLa nuclear cell extract by fluorescence polarization assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 5 — Multiple protein complex / RNA |
| Curated By | Autocuration |
Publication
Development of a fluorescence polarization based assay for histone deacetylase ligand discovery.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 6 | 7.75 | 8.82 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL99 | TRICHOSTATIN | 1.0 | 1 | 8.82 |
| CHEMBL430060 | APICIDIN | — | 1 | 8.10 |
| CHEMBL98 | VORINOSTAT | 4.0 | 1 | 7.62 |
| CHEMBL260653 | — | — | 1 | 7.24 |
| CHEMBL343448 | ROMIDEPSIN | 4.0 | 1 | 6.97 |
| CHEMBL86537 | SPLITOMICIN | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL99 | TRICHOSTATIN | IC50 | = | 1.5 | nM | 8.82 |
| CHEMBL430060 | APICIDIN | IC50 | = | 8.0 | nM | 8.10 |
| CHEMBL98 | VORINOSTAT | IC50 | = | 23.9 | nM | 7.62 |
| CHEMBL260653 | — | IC50 | = | 58.0 | nM | 7.24 |
| CHEMBL343448 | ROMIDEPSIN | IC50 | = | 107.2 | nM | 6.97 |
| CHEMBL86537 | SPLITOMICIN | IC50 | > | 75000.0 | nM | - |