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Assay Detail

CHEMBL940525

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of rat RBL2H3 cell degranulation assessed as inhibition of beta-hexosamidase release after 10 mins by microplate reader assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type RBL-2H3
Confidence 1 — Organism
Curated By Autocuration

Target

RBL-2H3 (CHEMBL614632)
Type CELL-LINE
Organism Rattus norvegicus

Publication

Absolute stereostructures of three new sesquiterpenes from the fruit of Alpinia oxyphylla with inhibitory effects on nitric oxide production and degranulation in RBL-2H3 cells.
Morikawa T, Matsuda H, Toguchida I, Ueda K, Yoshikawa M.
J Nat Prod (2002) 65 : 1468 -1474
PubMed 12398545 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 4.64 5.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL140 CURCUMIN 3.0 1 5.52
CHEMBL164841 TECHTOCHRYSIN 1 4.31
CHEMBL151 LUTEOLIN 2.0 1 4.09
CHEMBL415324 TRANILAST 3.0 1 -
CHEMBL1633 KETOTIFEN FUMARATE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL140 CURCUMIN IC50 = 3000.0 nM 5.52
CHEMBL164841 TECHTOCHRYSIN IC50 = 49000.0 nM 4.31
CHEMBL151 LUTEOLIN IC50 = 82000.0 nM 4.09
CHEMBL415324 TRANILAST IC50 = 490000.0 nM -
CHEMBL1633 KETOTIFEN FUMARATE IC50 = 220000.0 nM -