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Assay Detail

CHEMBL942384

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of human PPARgamma ligand binding domain-mediated transcriptional activity in human HepG2/C3A cells co-transfected with fused Gal4-LBD by cotransfection assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HepG2/C3A
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor gamma (CHEMBL235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Insights into the mechanism of partial agonism: crystal structures of the peroxisome proliferator-activated receptor gamma ligand-binding domain in the complex with two enantiomeric ligands.
Pochetti G, Godio C, Mitro N, Caruso D, Galmozzi A, Scurati S, Loiodice F, Fracchiolla G, Tortorella P, Laghezza A, Lavecchia A, Novellino E, Mazza F, Crestani M.
J Biol Chem (2007) 282 : 17314 -17324
PubMed 17403688 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 7.18 8.42

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL121 ROSIGLITAZONE 4.0 1 8.42
CHEMBL479207 1 7.13
CHEMBL477312 1 6.95
CHEMBL477119 1 6.23

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL121 ROSIGLITAZONE EC50 = 3.8 nM 8.42
CHEMBL479207 EC50 = 73.3 nM 7.13
CHEMBL477312 EC50 = 111.3 nM 6.95
CHEMBL477119 EC50 = 593.0 nM 6.23