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Assay Detail

CHEMBL947997

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human SGLT2 expressed in CHO cells assessed as inhibition of [14C]AMG accumulation
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium/glucose cotransporter 2 (CHEMBL3884)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of dapagliflozin: a potent, selective renal sodium-dependent glucose cotransporter 2 (SGLT2) inhibitor for the treatment of type 2 diabetes.
Meng W, Ellsworth BA, Nirschl AA, McCann PJ, Patel M, Girotra RN, Wu G, Sher PM, Morrison EP, Biller SA, Zahler R, Deshpande PP, Pullockaran A, Hagan DL, Morgan N, Taylor JR, Obermeier MT, Humphreys WG, Khanna A, Discenza L, Robertson JG, Wang A, Han S, Wetterau JR, Janovitz EB, Flint OP, Whaley JM, Washburn WN.
J Med Chem (2008) 51 : 1145 -1149
PubMed 18260618 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 7.70 8.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL429910 DAPAGLIFLOZIN 4.0 1 8.96
CHEMBL183937 1 8.18
CHEMBL270766 SERGLIFLOZIN A 1 8.04
CHEMBL245067 PHLORIZIN 1 7.45
CHEMBL429911 1 5.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL429910 DAPAGLIFLOZIN EC50 = 1.1 nM 8.96
CHEMBL183937 EC50 = 6.6 nM 8.18
CHEMBL270766 SERGLIFLOZIN A EC50 = 9.2 nM 8.04
CHEMBL245067 PHLORIZIN EC50 = 35.6 nM 7.45
CHEMBL429911 EC50 = 1300.0 nM 5.89