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Assay Detail

CHEMBL950044

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]nicotine from alpha4beta2 nAChR in Sprague-Dawley rat brain membrane
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Subcellular Membrane
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Deconstructing cytisine: The syntheses of (+/-)-cyfusine and (+/-)-cyclopropylcyfusine, fused ring analogs of cytisine.
Yohannes D, Procko K, Lebel LA, Fox CB, O'Neill BT.
Bioorg Med Chem Lett (2008) 18 : 2316 -2319
PubMed 18356044 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 8.00 9.37

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL497939 CYTISINICLINE 3.0 1 9.37
CHEMBL404660 CYFUSINE 1 7.80
CHEMBL401532 CYCLOPROPYLCYFUSINE 1 6.84

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL497939 CYTISINICLINE Ki = 0.43 nM 9.37
CHEMBL404660 CYFUSINE Ki = 16.0 nM 7.80
CHEMBL401532 CYCLOPROPYLCYFUSINE Ki = 144.0 nM 6.84