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Assay Detail

CHEMBL951599

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human CXCR4 I284A mutant expressed in COS7 cells coexpressing G protein Gqi4myr assessed as inhibition of CXCL12-induced phosphatidylinositol turnover
8
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type COS-7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

C-X-C chemokine receptor type 4 (CHEMBL2107)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Molecular mechanism of action of monocyclam versus bicyclam non-peptide antagonists in the CXCR4 chemokine receptor.
Rosenkilde MM, Gerlach LO, Hatse S, Skerlj RT, Schols D, Bridger GJ, Schwartz TW.
J Biol Chem (2007) 282 : 27354 -27365
PubMed 17599916 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
logIC50 4 - -
Ki 4 6.37 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL477121 2 7.52
CHEMBL478168 2 6.38
CHEMBL18442 PLERIXAFOR 4.0 2 5.92
CHEMBL122226 2 5.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL477121 Ki = 30.0 nM 7.52
CHEMBL478168 Ki = 420.0 nM 6.38
CHEMBL18442 PLERIXAFOR Ki = 1200.0 nM 5.92
CHEMBL122226 Ki = 2300.0 nM 5.64
CHEMBL18442 PLERIXAFOR logIC50 = -5.94 -
CHEMBL122226 logIC50 = -5.64 -
CHEMBL478168 logIC50 = -6.38 -
CHEMBL477121 logIC50 = -7.5 -