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Assay Detail

CHEMBL952768

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HIV1 LAI reverse transcriptase wild type in presence of 5 mM ATP by heteropolymeric DNA polymerase assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Human immunodeficiency virus 1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Human immunodeficiency virus type 1 reverse transcriptase (CHEMBL247)
Type SINGLE PROTEIN
Organism Human immunodeficiency virus 1

Publication

Biochemical studies on the mechanism of human immunodeficiency virus type 1 reverse transcriptase resistance to 1-(beta-D-dioxolane)thymine triphosphate.
Lennerstrand J, Chu CK, Schinazi RF.
Antimicrob Agents Chemother (2007) 51 : 2078 -2084
PubMed 17403997 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 6.38 6.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL258936 1 6.72
CHEMBL261773 1 6.70
CHEMBL193952 ZIDOVUDINE TRIPHOSPHATE -1.0 1 6.52
CHEMBL1201395 CARBOVIR 5'-TRIPHOSPHATE, (-)- -1.0 1 6.05
CHEMBL1236349 1 5.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL258936 IC50 = 190.0 nM 6.72
CHEMBL261773 IC50 = 200.0 nM 6.70
CHEMBL193952 ZIDOVUDINE TRIPHOSPHATE IC50 = 300.0 nM 6.52
CHEMBL1201395 CARBOVIR 5'-TRIPHOSPHATE, (-)- IC50 = 900.0 nM 6.05
CHEMBL1236349 IC50 = 1300.0 nM 5.89