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Assay Detail

CHEMBL953930

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Protection against Bacillus anthracis lethal toxin-mediated cytotoxicity in mouse RAW264.7 cells assessed as change in viability after 24 hrs by WST1 dye reduction assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bacillus anthracis
Cell Type RAW264.7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lethal factor (CHEMBL4372)
Type SINGLE PROTEIN
Organism Bacillus anthracis

Publication

Amiodarone and bepridil inhibit anthrax toxin entry into host cells.
Sanchez AM, Thomas D, Gillespie EJ, Damoiseaux R, Rogers J, Saxe JP, Huang J, Manchester M, Bradley KA.
Antimicrob Agents Chemother (2007) 51 : 2403 -2411
PubMed 17485504 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.56 5.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL7463 GF-109203 1 5.82
CHEMBL322333 DIMETHYLSPINGOSINE 1 5.64
CHEMBL633 AMIODARONE 4.0 1 5.46
CHEMBL1008 BEPRIDIL 4.0 1 5.32

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL7463 GF-109203 IC50 = 1500.0 nM 5.82
CHEMBL322333 DIMETHYLSPINGOSINE IC50 = 2300.0 nM 5.64
CHEMBL633 AMIODARONE IC50 = 3500.0 nM 5.46
CHEMBL1008 BEPRIDIL IC50 = 4800.0 nM 5.32