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Assay Detail

CHEMBL954009

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant Nav1.5 channel expressed in HEK293 cells by whole cell voltage clamp technique
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium channel protein type 5 subunit alpha (CHEMBL1980)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of potent furan piperazine sodium channel blockers for treatment of neuropathic pain.
Drizin I, Gregg RJ, Scanio MJ, Shi L, Gross MF, Atkinson RN, Thomas JB, Johnson MS, Carroll WA, Marron BE, Chapman ML, Liu D, Krambis MJ, Shieh CC, Zhang X, Hernandez G, Gauvin DM, Mikusa JP, Zhu CZ, Joshi S, Honore P, Marsh KC, Roeloffs R, Werness S, Krafte DS, Jarvis MF, Faltynek CR, Kort ME.
Bioorg Med Chem (2008) 16 : 6379 -6386
PubMed 18501613 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.42 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL486495 1 7.00
CHEMBL482676 1 5.64
CHEMBL521211 1 5.34
CHEMBL558 MEXILETINE 4.0 1 4.89
CHEMBL741 LAMOTRIGINE 4.0 1 4.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL486495 IC50 = 100.0 nM 7.00
CHEMBL482676 IC50 = 2300.0 nM 5.64
CHEMBL521211 IC50 = 4600.0 nM 5.34
CHEMBL558 MEXILETINE IC50 = 13000.0 nM 4.89
CHEMBL741 LAMOTRIGINE IC50 = 62000.0 nM 4.21