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Assay Detail

CHEMBL954343

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human recombinant GnRH receptor expressed in HEK293 cells assessed as reduction in (D-Trp6)-GnRH-stimulated IP production by whole cell assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Gonadotropin-releasing hormone receptor (CHEMBL1855)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Small molecule antagonists of the gonadotropin-releasing hormone (GnRH) receptor: structure-activity relationships of small heterocyclic groups appended to the 2-phenyl-4-piperazinyl-benzimidazole template.
Hauze DB, Chengalvala MV, Cottom JE, Feingold IB, Garrick L, Green DM, Huselton C, Kao W, Kees K, Lundquist JT, Mann CW, Mehlmann JF, Rogers JF, Shanno L, Wrobel J, Pelletier JC.
Bioorg Med Chem Lett (2009) 19 : 1986 -1990
PubMed 19251413 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 7.67 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL460480 1 8.40
CHEMBL493731 1 7.89
CHEMBL521674 1 7.85
CHEMBL492327 1 7.64
CHEMBL493344 1 7.37
CHEMBL494400 1 7.36
CHEMBL493565 1 7.15

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL460480 IC50 = 4.0 nM 8.40
CHEMBL493731 IC50 = 13.0 nM 7.89
CHEMBL521674 IC50 = 14.0 nM 7.85
CHEMBL492327 IC50 = 23.0 nM 7.64
CHEMBL493344 IC50 = 43.0 nM 7.37
CHEMBL494400 IC50 = 44.0 nM 7.36
CHEMBL493565 IC50 = 71.0 nM 7.15