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Assay Detail

CHEMBL954625

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]flumazenil from rat GABA-A alpha-1-beta-2-gamma-2 receptor expressed in HEK293 cells
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

GABA-A receptor; alpha-1/beta-2/gamma-2 (CHEMBL2095167)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Identification of anxiolytic/nonsedative agents among indol-3-ylglyoxylamides acting as functionally selective agonists at the gamma-aminobutyric acid-A (GABAA) alpha2 benzodiazepine receptor.
Taliani S, Cosimelli B, Da Settimo F, Marini AM, La Motta C, Simorini F, Salerno S, Novellino E, Greco G, Cosconati S, Marinelli L, Salvetti F, L'Abbate G, Trasciatti S, Montali M, Costa B, Martini C.
J Med Chem (2009) 52 : 3723 -3734
PubMed 19469479 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 20 7.00 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL521901 1 8.05
CHEMBL12 DIAZEPAM 4.0 1 7.96
CHEMBL75456 1 7.85
CHEMBL74302 1 7.80
CHEMBL495634 1 7.55
CHEMBL75306 1 7.38
CHEMBL911 ZOLPIDEM 4.0 1 7.30
CHEMBL306310 1 6.71
CHEMBL495615 1 6.71
CHEMBL495616 1 6.70
CHEMBL495592 1 6.65
CHEMBL127135 1 6.61
CHEMBL495635 1 6.61
CHEMBL307139 1 6.53
CHEMBL75406 1 5.94
CHEMBL522716 1 5.69
CHEMBL496228 1 -
CHEMBL522059 1 -
CHEMBL522888 1 -
CHEMBL339918 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL521901 Ki = 9.0 nM 8.05
CHEMBL12 DIAZEPAM Ki = 11.0 nM 7.96
CHEMBL75456 Ki = 14.0 nM 7.85
CHEMBL74302 Ki = 16.0 nM 7.80
CHEMBL495634 Ki = 28.0 nM 7.55
CHEMBL75306 Ki = 42.0 nM 7.38
CHEMBL911 ZOLPIDEM Ki = 50.0 nM 7.30
CHEMBL306310 Ki = 195.0 nM 6.71
CHEMBL495615 Ki = 195.0 nM 6.71
CHEMBL495616 Ki = 200.0 nM 6.70
CHEMBL495592 Ki = 225.0 nM 6.65
CHEMBL127135 Ki = 245.0 nM 6.61
CHEMBL495635 Ki = 246.0 nM 6.61
CHEMBL307139 Ki = 293.0 nM 6.53
CHEMBL75406 Ki = 1150.0 nM 5.94
CHEMBL522716 Ki = 2049.0 nM 5.69
CHEMBL496228 Ki - -
CHEMBL522059 Ki - -
CHEMBL522888 Ki - -
CHEMBL339918 Ki - -