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Assay Detail

CHEMBL960265

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]ryanodine from RyR1 calcium channel in sarcoplasmic reticulum assessed as calcium mobilization
5
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Intermediate

Target

Ryanodine receptor 1 (CHEMBL1846)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Cationic reduction of bastadin-4 to bastadin-5. Preparation of 5-[2h]-bastadin-5 by site-specific isotopic labeling.
Masuno MN, Molinski TF.
J Nat Prod (2003) 66 : 112 -114
PubMed 12542356 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 5.22 5.58
ED50 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL505907 1 5.58
CHEMBL504851 1 5.24
CHEMBL455487 BASTADIN 4 1 4.83
CHEMBL439151 BASTADIN 5 1 -
CHEMBL508830 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL505907 EC50 = 2600.0 nM 5.58
CHEMBL504851 EC50 = 5800.0 nM 5.24
CHEMBL455487 BASTADIN 4 EC50 = 14700.0 nM 4.83
CHEMBL439151 BASTADIN 5 ED50 = 2.3 uM -
CHEMBL508830 EC50 > 100000.0 nM -