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Assay Detail

CHEMBL963129

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of MEK-mediated ERK phosphorylation in PDGF-stimulated mouse K-Balb cells treated as single oral dose preincubated for 1 hr before PDGF challenge by whole cell assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

The discovery of the benzhydroxamate MEK inhibitors CI-1040 and PD 0325901.
Barrett SD, Bridges AJ, Dudley DT, Saltiel AR, Fergus JH, Flamme CM, Delaney AM, Kaufman M, LePage S, Leopold WR, Przybranowski SA, Sebolt-Leopold J, Van Becelaere K, Doherty AM, Kennedy RM, Marston D, Howard WA, Smith Y, Warmus JS, Tecle H.
Bioorg Med Chem Lett (2008) 18 : 6501 -6504
PubMed 18952427 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 7.80 8.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL510244 1 8.80
CHEMBL105442 CI-1040 2.0 1 8.61
CHEMBL511056 1 8.45
CHEMBL442641 1 8.38
CHEMBL454880 1 8.36
CHEMBL453890 1 8.19
CHEMBL455470 1 8.09
CHEMBL448323 1 7.93
CHEMBL455471 1 7.63
CHEMBL454114 1 7.58
CHEMBL455472 1 7.26
CHEMBL510625 1 7.12
CHEMBL454115 1 6.92
CHEMBL454116 1 5.94

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL510244 IC50 = 1.59 nM 8.80
CHEMBL105442 CI-1040 IC50 = 2.45 nM 8.61
CHEMBL511056 IC50 = 3.55 nM 8.45
CHEMBL442641 IC50 = 4.18 nM 8.38
CHEMBL454880 IC50 = 4.42 nM 8.36
CHEMBL453890 IC50 = 6.48 nM 8.19
CHEMBL455470 IC50 = 8.18 nM 8.09
CHEMBL448323 IC50 = 11.8 nM 7.93
CHEMBL455471 IC50 = 23.5 nM 7.63
CHEMBL454114 IC50 = 26.4 nM 7.58
CHEMBL455472 IC50 = 54.9 nM 7.26
CHEMBL510625 IC50 = 75.8 nM 7.12
CHEMBL454115 IC50 = 119.0 nM 6.92
CHEMBL454116 IC50 = 1140.0 nM 5.94