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Assay Detail

CHEMBL963253

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]cytisine from alpha4beta2 nAChR in rat brain membrane
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Subcellular Membrane
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Preparation and characterization of N-(3-pyridinyl) spirocyclic diamines as ligands for nicotinic acetylcholine receptors.
Sippy KB, Anderson DJ, Bunnelle WH, Hutchins CW, Schrimpf MR.
Bioorg Med Chem Lett (2009) 19 : 1682 -1685
PubMed 19232492 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 11 8.42 10.33

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL6623 EPIBATIDINE 1 10.33
CHEMBL430497 TEBANICLINE 2.0 1 10.32
CHEMBL389964 1 9.82
CHEMBL3 NICOTINE 4.0 1 9.03
CHEMBL494773 1 8.92
CHEMBL449142 1 7.96
CHEMBL492593 1 7.68
CHEMBL504652 TC-2216 1.0 1 7.48
CHEMBL509239 1 7.32
CHEMBL504595 1 7.13
CHEMBL504126 1 6.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL6623 EPIBATIDINE Ki = 0.047 nM 10.33
CHEMBL430497 TEBANICLINE Ki = 0.048 nM 10.32
CHEMBL389964 Ki = 0.15 nM 9.82
CHEMBL3 NICOTINE Ki = 0.94 nM 9.03
CHEMBL494773 Ki = 1.2 nM 8.92
CHEMBL449142 Ki = 11.0 nM 7.96
CHEMBL492593 Ki = 21.0 nM 7.68
CHEMBL504652 TC-2216 Ki = 33.0 nM 7.48
CHEMBL509239 Ki = 48.0 nM 7.32
CHEMBL504595 Ki = 74.0 nM 7.13
CHEMBL504126 Ki = 240.0 nM 6.62