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Assay Detail

CHEMBL964079

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Functional
Agonist activity at human recombinant alpha4beta2 nAChR expressed in human IMR32 cells assessed as calcium dynamics by FLIPR assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type IMR-32
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907589)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Preparation and characterization of N-(3-pyridinyl) spirocyclic diamines as ligands for nicotinic acetylcholine receptors.
Sippy KB, Anderson DJ, Bunnelle WH, Hutchins CW, Schrimpf MR.
Bioorg Med Chem Lett (2009) 19 : 1682 -1685
PubMed 19232492 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 11 5.99 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL430497 TEBANICLINE 2.0 1 7.70
CHEMBL6623 EPIBATIDINE 1 6.92
CHEMBL494773 1 5.80
CHEMBL492593 1 5.60
CHEMBL3 NICOTINE 4.0 1 5.33
CHEMBL449142 1 4.60
CHEMBL389964 1 -
CHEMBL509239 1 -
CHEMBL504652 TC-2216 1.0 1 -
CHEMBL504126 1 -
CHEMBL504595 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL430497 TEBANICLINE EC50 = 20.0 nM 7.70
CHEMBL6623 EPIBATIDINE EC50 = 120.0 nM 6.92
CHEMBL494773 EC50 = 1600.0 nM 5.80
CHEMBL492593 EC50 = 2500.0 nM 5.60
CHEMBL3 NICOTINE EC50 = 4700.0 nM 5.33
CHEMBL449142 EC50 = 25000.0 nM 4.60
CHEMBL389964 EC50 - -
CHEMBL509239 EC50 > 100000.0 nM -
CHEMBL504652 TC-2216 EC50 > 100000.0 nM -
CHEMBL504126 EC50 > 100000.0 nM -
CHEMBL504595 EC50 > 100000.0 nM -