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Assay Detail

CHEMBL971431

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human P2Y2 receptor expressed in human 1321N1 cells coexpressing phospholipase C-activating G protein assessed as inositol phosphate production by scintillation proximity assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type 1321-N1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

P2Y purinoceptor 2 (CHEMBL4398)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and P2Y receptor activity of nucleoside 5'-phosphonate derivatives.
Cosyn L, Van Calenbergh S, Joshi BV, Ko H, Carter RL, Kendall Harden T, Jacobson KA.
Bioorg Med Chem Lett (2009) 19 : 3002 -3005
PubMed 19419868 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 5.88 7.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL14249 ADENOSINE TRIPHOSPHATE 2.0 1 7.07
CHEMBL523850 1 6.00
CHEMBL493370 1 5.85
CHEMBL522015 1 5.80
CHEMBL505857 1 5.47
CHEMBL493371 1 5.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL14249 ADENOSINE TRIPHOSPHATE EC50 = 85.0 nM 7.07
CHEMBL523850 EC50 = 1000.0 nM 6.00
CHEMBL493370 EC50 = 1400.0 nM 5.85
CHEMBL522015 EC50 = 1600.0 nM 5.80
CHEMBL505857 EC50 = 3400.0 nM 5.47
CHEMBL493371 EC50 = 8600.0 nM 5.07