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Assay Detail

CHEMBL975203

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of cholesterol synthesis in rat hepatocytes assessed as incorporation of [14C]acetate into cholesterol
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type Hepatocyte
Confidence 1 — Organism
Curated By Autocuration

Target

Hepatocyte (CHEMBL613362)
Type CELL-LINE
Organism Rattus norvegicus

Publication

(3R,5S,E)-7-(4-(4-fluorophenyl)-6-isopropyl-2-(methyl(1-methyl-1h-1,2,4-triazol-5-yl)amino)pyrimidin-5-yl)-3,5-dihydroxyhept-6-enoic acid (BMS-644950): a rationally designed orally efficacious 3-hydroxy-3-methylglutaryl coenzyme-a reductase inhibitor with reduced myotoxicity potential.
Ahmad S, Madsen CS, Stein PD, Janovitz E, Huang C, Ngu K, Bisaha S, Kennedy LJ, Chen BC, Zhao R, Sitkoff D, Monshizadegan H, Yin X, Ryan CS, Zhang R, Giancarli M, Bird E, Chang M, Chen X, Setters R, Search D, Zhuang S, Nguyen-Tran V, Cuff CA, Harrity T, Darienzo CJ, Li T, Reeves RA, Blanar MA, Barrish JC, Zahler R, Robl JA.
J Med Chem (2008) 51 : 2722 -2733
PubMed 18412317 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 8.47 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1496 ROSUVASTATIN 4.0 1 9.22
CHEMBL468631 1 9.00
CHEMBL468768 1 8.92
CHEMBL498360 1 8.89
CHEMBL466547 1 8.85
CHEMBL511255 1 8.85
CHEMBL466546 1 8.82
CHEMBL468204 1 8.72
CHEMBL1477 CERIVASTATIN 4.0 1 8.64
CHEMBL1487 ATORVASTATIN 4.0 1 8.60
CHEMBL525188 1 8.60
CHEMBL467782 1 8.43
CHEMBL468205 1 8.43
CHEMBL466326 1 8.40
CHEMBL495463 1 8.27
CHEMBL498161 1 8.26
CHEMBL1064 SIMVASTATIN 4.0 1 8.21
CHEMBL511574 1 8.12
CHEMBL466352 1 8.11
CHEMBL468862 1 8.11
CHEMBL496100 1 8.05
CHEMBL468632 1 7.86
CHEMBL1144 PRAVASTATIN 4.0 1 7.54

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1496 ROSUVASTATIN IC50 = 0.6 nM 9.22
CHEMBL468631 IC50 = 1.0 nM 9.00
CHEMBL468768 IC50 = 1.2 nM 8.92
CHEMBL498360 IC50 = 1.3 nM 8.89
CHEMBL466547 IC50 = 1.4 nM 8.85
CHEMBL511255 IC50 = 1.4 nM 8.85
CHEMBL466546 IC50 = 1.5 nM 8.82
CHEMBL468204 IC50 = 1.9 nM 8.72
CHEMBL1477 CERIVASTATIN IC50 = 2.3 nM 8.64
CHEMBL1487 ATORVASTATIN IC50 = 2.5 nM 8.60
CHEMBL525188 IC50 = 2.5 nM 8.60
CHEMBL467782 IC50 = 3.7 nM 8.43
CHEMBL468205 IC50 = 3.7 nM 8.43
CHEMBL466326 IC50 = 3.95 nM 8.40
CHEMBL495463 IC50 = 5.4 nM 8.27
CHEMBL498161 IC50 = 5.5 nM 8.26
CHEMBL1064 SIMVASTATIN IC50 = 6.2 nM 8.21
CHEMBL511574 IC50 = 7.5 nM 8.12
CHEMBL466352 IC50 = 7.7 nM 8.11
CHEMBL468862 IC50 = 7.7 nM 8.11
CHEMBL496100 IC50 = 9.0 nM 8.05
CHEMBL468632 IC50 = 13.7 nM 7.86
CHEMBL1144 PRAVASTATIN IC50 = 29.0 nM 7.54