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Assay Detail

CHEMBL979852

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]substance P from human recombinant NK1 receptor expressed in CHO cells
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Substance-P receptor (CHEMBL249)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery process and pharmacological characterization of 2-(S)-(4-fluoro-2-methylphenyl)piperazine-1-carboxylic acid [1-(R)-(3,5-bis-trifluoromethylphenyl)ethyl]methylamide (vestipitant) as a potent, selective, and orally active NK1 receptor antagonist.
Di Fabio R, Griffante C, Alvaro G, Pentassuglia G, Pizzi DA, Donati D, Rossi T, Guercio G, Mattioli M, Cimarosti Z, Marchioro C, Provera S, Zonzini L, Montanari D, Melotto S, Gerrard PA, Trist DG, Ratti E, Corsi M.
J Med Chem (2009) 52 : 3238 -3247
PubMed 19388677 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 9 8.87 10.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL522302 VOFOPITANT 2.0 1 10.30
CHEMBL522987 VESTIPITANT 2.0 1 9.65
CHEMBL490926 1 9.37
CHEMBL492117 1 9.27
CHEMBL490755 1 8.89
CHEMBL523156 1 8.43
CHEMBL489924 1 8.09
CHEMBL521605 1 8.06
CHEMBL522292 1 7.74

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL522302 VOFOPITANT Ki = 0.05012 nM 10.30
CHEMBL522987 VESTIPITANT Ki = 0.2239 nM 9.65
CHEMBL490926 Ki = 0.4266 nM 9.37
CHEMBL492117 Ki = 0.537 nM 9.27
CHEMBL490755 Ki = 1.288 nM 8.89
CHEMBL523156 Ki = 3.715 nM 8.43
CHEMBL489924 Ki = 8.128 nM 8.09
CHEMBL521605 Ki = 8.71 nM 8.06
CHEMBL522292 Ki = 18.2 nM 7.74