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Assay Detail

CHEMBL984568

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at estrogen receptor alpha expressed in human HEC1 cells assessed as inhibition of 1 nM estradiol-induced transcriptional activity after 24 hrs by luciferase reporter gene assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Cell Type HEC-1
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Gag polyprotein (CHEMBL5824)
Type SINGLE PROTEIN
Organism Avian erythroblastosis virus (strain ES4)

Publication

Synthesis and biological evaluation of guanylhydrazone coactivator binding inhibitors for the estrogen receptor.
LaFrate AL, Gunther JR, Carlson KE, Katzenellenbogen JA.
Bioorg Med Chem (2008) 16 : 10075 -10084
PubMed 18976929 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 5.55 6.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL458496 1 6.05
CHEMBL456392 1 5.92
CHEMBL456598 1 5.89
CHEMBL448651 1 5.85
CHEMBL460174 1 5.85
CHEMBL456393 1 5.82
CHEMBL456106 1 5.70
CHEMBL456600 1 5.51
CHEMBL456594 1 5.48
CHEMBL512206 1 5.46
CHEMBL456179 1 5.41
CHEMBL33663 1 5.12
CHEMBL285853 1 5.12
CHEMBL456599 1 5.11
CHEMBL458495 1 4.92
CHEMBL459968 1 -
CHEMBL508968 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL458496 IC50 = 900.0 nM 6.05
CHEMBL456392 IC50 = 1200.0 nM 5.92
CHEMBL456598 IC50 = 1300.0 nM 5.89
CHEMBL448651 IC50 = 1400.0 nM 5.85
CHEMBL460174 IC50 = 1400.0 nM 5.85
CHEMBL456393 IC50 = 1500.0 nM 5.82
CHEMBL456106 IC50 = 2000.0 nM 5.70
CHEMBL456600 IC50 = 3100.0 nM 5.51
CHEMBL456594 IC50 = 3300.0 nM 5.48
CHEMBL512206 IC50 = 3500.0 nM 5.46
CHEMBL456179 IC50 = 3900.0 nM 5.41
CHEMBL33663 IC50 = 7500.0 nM 5.12
CHEMBL285853 IC50 = 7500.0 nM 5.12
CHEMBL456599 IC50 = 7800.0 nM 5.11
CHEMBL458495 IC50 = 12000.0 nM 4.92
CHEMBL459968 IC50 >= 50000.0 nM -
CHEMBL508968 IC50 >= 50000.0 nM -