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Assay Detail

CHEMBL986430

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Binding
Inhibition of Mycobacterium avium DHFR using dihydrofolic acid substrate and NADPH cofactor
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mycobacterium avium
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

The effect of 5-alkyl modification on the biological activity of pyrrolo[2,3-d]pyrimidine containing classical and nonclassical antifolates as inhibitors of dihydrofolate reductase and as antitumor and/or antiopportunistic infection agents.
Gangjee A, Jain HD, Queener SF, Kisliuk RL.
J Med Chem (2008) 51 : 4589 -4600
PubMed 18605720 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 24 6.31 9.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL476751 1 9.41
CHEMBL476548 1 9.13
CHEMBL119 TRIMETREXATE 4.0 1 8.82
CHEMBL331986 1 7.00
CHEMBL227009 1 6.98
CHEMBL476745 1 6.68
CHEMBL476945 1 6.62
CHEMBL22 TRIMETHOPRIM 4.0 1 6.52
CHEMBL478403 1 6.43
CHEMBL476744 1 6.22
CHEMBL477790 1 6.20
CHEMBL389686 1 6.06
CHEMBL477151 1 5.94
CHEMBL516891 1 5.88
CHEMBL516128 1 5.68
CHEMBL477150 1 5.59
CHEMBL478191 1 5.57
CHEMBL477566 1 5.47
CHEMBL515312 1 5.39
CHEMBL478213 1 5.11
CHEMBL477789 1 4.98
CHEMBL514219 1 4.91
CHEMBL478192 1 4.54
CHEMBL478404 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL476751 IC50 = 0.391 nM 9.41
CHEMBL476548 IC50 = 0.737 nM 9.13
CHEMBL119 TRIMETREXATE IC50 = 1.5 nM 8.82
CHEMBL331986 IC50 = 100.0 nM 7.00
CHEMBL227009 IC50 = 104.3 nM 6.98
CHEMBL476745 IC50 = 210.0 nM 6.68
CHEMBL476945 IC50 = 240.0 nM 6.62
CHEMBL22 TRIMETHOPRIM IC50 = 300.0 nM 6.52
CHEMBL478403 IC50 = 368.7 nM 6.43
CHEMBL476744 IC50 = 600.0 nM 6.22
CHEMBL477790 IC50 = 630.0 nM 6.20
CHEMBL389686 IC50 = 880.0 nM 6.06
CHEMBL477151 IC50 = 1140.0 nM 5.94
CHEMBL516891 IC50 = 1310.0 nM 5.88
CHEMBL516128 IC50 = 2100.0 nM 5.68
CHEMBL477150 IC50 = 2540.0 nM 5.59
CHEMBL478191 IC50 = 2700.0 nM 5.57
CHEMBL477566 IC50 = 3400.0 nM 5.47
CHEMBL515312 IC50 = 4100.0 nM 5.39
CHEMBL478213 IC50 = 7800.0 nM 5.11
CHEMBL477789 IC50 = 10400.0 nM 4.98
CHEMBL514219 IC50 = 12300.0 nM 4.91
CHEMBL478192 IC50 = 28600.0 nM 4.54
CHEMBL478404 IC50 - -