Assay Detail
Binding
CHEMBL986820
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Activation of mouse wild type TRPV4 expressed in HEK293 cells assessed as increase in intracellular calcium concentration
11
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Transient receptor potential cation channel subfamily V member 4 (CHEMBL6126) ↗| Type | SINGLE PROTEIN |
| Organism | Mus musculus |
Publication
Modulation of the transient receptor potential vanilloid channel TRPV4 by 4alpha-phorbol esters: a structure-activity study.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 10 | 6.15 | 7.16 |
| Activity | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL490470 | — | — | 2 | 7.16 |
| CHEMBL504955 | — | — | 1 | 6.76 |
| CHEMBL118987 | — | — | 1 | 6.43 |
| CHEMBL541720 | — | — | 1 | 6.35 |
| CHEMBL451849 | — | — | 1 | 5.55 |
| CHEMBL27768 | — | — | 1 | 4.65 |
| CHEMBL484343 | — | — | 1 | - |
| CHEMBL507303 | — | — | 1 | - |
| CHEMBL130626 | — | — | 1 | - |
| CHEMBL504854 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL490470 | — | EC50 | = | 70.0 | nM | 7.16 |
| CHEMBL504955 | — | EC50 | = | 175.0 | nM | 6.76 |
| CHEMBL118987 | — | EC50 | = | 370.0 | nM | 6.43 |
| CHEMBL541720 | — | EC50 | = | 450.0 | nM | 6.35 |
| CHEMBL451849 | — | EC50 | = | 2830.0 | nM | 5.55 |
| CHEMBL27768 | — | EC50 | = | 22470.0 | nM | 4.65 |
| CHEMBL484343 | — | EC50 | > | 50000.0 | nM | - |
| CHEMBL507303 | — | EC50 | > | 50000.0 | nM | - |
| CHEMBL130626 | — | EC50 | > | 50000.0 | nM | - |
| CHEMBL504854 | — | EC50 | > | 50000.0 | nM | - |
| CHEMBL490470 | — | Activity | - | — | - |