Assay Detail
Binding
CHEMBL987100
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of GLI1-mediated transcriptional activity in human HaCaT cells by luciferase based reporter gene assay
8
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HaCaT |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Hedgehog/GLI-mediated transcriptional inhibitors from Zizyphus cambodiana.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 4.43 | 4.50 |
| Activity | 3 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL269277 | BETULINIC ACID | 1.0 | 2 | 4.50 |
| CHEMBL470503 | COLUBRINIC ACID | — | 2 | 4.42 |
| CHEMBL511805 | ALPHITOLIC ACID | — | 2 | 4.38 |
| CHEMBL491069 | CEANOTHIC ACID | — | 1 | - |
| CHEMBL522108 | CEANOTHANOLIC ACID | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL269277 | BETULINIC ACID | IC50 | = | 32000.0 | nM | 4.50 |
| CHEMBL470503 | COLUBRINIC ACID | IC50 | = | 38000.0 | nM | 4.42 |
| CHEMBL511805 | ALPHITOLIC ACID | IC50 | = | 42000.0 | nM | 4.38 |
| CHEMBL491069 | CEANOTHIC ACID | IC50 | > | 200000.0 | nM | - |
| CHEMBL522108 | CEANOTHANOLIC ACID | IC50 | = | 133000.0 | nM | - |
| CHEMBL470503 | COLUBRINIC ACID | Activity | - | — | - | |
| CHEMBL269277 | BETULINIC ACID | Activity | - | — | - | |
| CHEMBL511805 | ALPHITOLIC ACID | Activity | - | — | - |