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Assay Detail

CHEMBL990114

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of GST fused human liver glucokinase assessed as generation of NADPH by G6PDH coupled assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hexokinase-4 (CHEMBL3820)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

SAR, pharmacokinetics, safety, and efficacy of glucokinase activating 2-(4-sulfonylphenyl)-N-thiazol-2-ylacetamides: discovery of PSN-GK1.
Bertram LS, Black D, Briner PH, Chatfield R, Cooke A, Fyfe MC, Murray PJ, Naud F, Nawano M, Procter MJ, Rakipovski G, Rasamison CM, Reynet C, Schofield KL, Shah VK, Spindler F, Taylor A, Turton R, Williams GM, Wong-Kai-In P, Yasuda K.
J Med Chem (2008) 51 : 4340 -4345
PubMed 18588279 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 5.93 7.16

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL480725 1 7.16
CHEMBL499520 1 6.89
CHEMBL519273 1 6.85
CHEMBL516458 1 6.25
CHEMBL479947 1 6.24
CHEMBL517534 1 6.24
CHEMBL479948 1 5.84
CHEMBL481166 1 5.64
CHEMBL481167 1 5.46
CHEMBL519934 1 5.05
CHEMBL479974 1 4.97
CHEMBL480180 1 4.58
CHEMBL479753 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL480725 EC50 = 70.0 nM 7.16
CHEMBL499520 EC50 = 130.0 nM 6.89
CHEMBL519273 EC50 = 140.0 nM 6.85
CHEMBL516458 EC50 = 560.0 nM 6.25
CHEMBL479947 EC50 = 580.0 nM 6.24
CHEMBL517534 EC50 = 570.0 nM 6.24
CHEMBL479948 EC50 = 1430.0 nM 5.84
CHEMBL481166 EC50 = 2300.0 nM 5.64
CHEMBL481167 EC50 = 3470.0 nM 5.46
CHEMBL519934 EC50 = 8930.0 nM 5.05
CHEMBL479974 EC50 = 10770.0 nM 4.97
CHEMBL480180 EC50 = 26010.0 nM 4.58
CHEMBL479753 EC50 > 10000.0 nM -