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Assay Detail

CHEMBL992544

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human ITK expressed in chicken DT40 cells assessed as B cell receptor-stimulated calcium influx by FLIPR assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type DT40
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase ITK/TSK (CHEMBL2959)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Itk kinase inhibitors: initial efforts to improve the metabolical stability and the cell activity of the benzimidazole lead.
Moriarty KJ, Winters M, Qiao L, Ryan D, DesJarlis R, Robinson D, Cook BN, Kashem MA, Kaplita PV, Liu LH, Farrell TM, Khine HH, King J, Pullen SS, Roth GP, Magolda R, Takahashi H.
Bioorg Med Chem Lett (2008) 18 : 5537 -5540
PubMed 18819794 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 6.24 6.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL462301 1 6.80
CHEMBL484766 1 6.58
CHEMBL485556 1 6.52
CHEMBL483581 1 6.48
CHEMBL460824 1 6.47
CHEMBL485203 1 6.47
CHEMBL484945 1 6.43
CHEMBL490269 1 6.30
CHEMBL485030 1 6.14
CHEMBL485626 1 5.96
CHEMBL519033 1 5.64
CHEMBL485201 1 5.14

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL462301 IC50 = 160.0 nM 6.80
CHEMBL484766 IC50 = 260.0 nM 6.58
CHEMBL485556 IC50 = 300.0 nM 6.52
CHEMBL483581 IC50 = 330.0 nM 6.48
CHEMBL460824 IC50 = 340.0 nM 6.47
CHEMBL485203 IC50 = 340.0 nM 6.47
CHEMBL484945 IC50 = 370.0 nM 6.43
CHEMBL490269 IC50 = 500.0 nM 6.30
CHEMBL485030 IC50 = 720.0 nM 6.14
CHEMBL485626 IC50 = 1100.0 nM 5.96
CHEMBL519033 IC50 = 2300.0 nM 5.64
CHEMBL485201 IC50 = 7200.0 nM 5.14