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Assay Detail

CHEMBL993554

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human cdc2/cyclin B assessed as [32P] incorporation in histone H1 from [gamma32P]ATP
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Cyclin-dependent kinase 1/cyclin B1 (CHEMBL1907602)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Exploiting chemical libraries, structure, and genomics in the search for kinase inhibitors.
Gray NS, Wodicka L, Thunnissen AM, Norman TC, Kwon S, Espinoza FH, Morgan DO, Barnes G, LeClerc S, Meijer L, Kim SH, Lockhart DJ, Schultz PG.
Science (1998) 281 : 533 -538
PubMed 9677190 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 7.70 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL23327 PURVALANOLA 1 8.40
CHEMBL23254 PURVALANOL B 1 8.22
CHEMBL311228 1 6.47

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL23327 PURVALANOLA IC50 = 4.0 nM 8.40
CHEMBL23254 PURVALANOL B IC50 = 6.0 nM 8.22
CHEMBL311228 IC50 = 340.0 nM 6.47