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Assay Detail

CHEMBL993724

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of [3H]glycine uptake at human glycine transporter 1 expressed in human JAR cells after 3 hrs by scintillation counting
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type JAR
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium- and chloride-dependent glycine transporter 1 (CHEMBL2337)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimisation of a series of potent, selective and orally bioavailable GlyT1 inhibitors.
Thomson JL, Blackaby WP, Jennings AS, Goodacre SC, Pike A, Thomas S, Brown TA, Smith A, Pillai G, Street LJ, Lewis RT.
Bioorg Med Chem Lett (2009) 19 : 2235 -2239
PubMed 19318248 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 7.94 10.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL485356 1 10.00
CHEMBL483938 1 9.51
CHEMBL521463 1 9.19
CHEMBL482764 1 8.76
CHEMBL482960 1 8.72
CHEMBL484734 1 8.70
CHEMBL483344 1 8.59
CHEMBL521182 1 8.53
CHEMBL485355 1 8.44
CHEMBL484733 1 8.36
CHEMBL483157 1 8.27
CHEMBL520648 1 8.26
CHEMBL482974 1 7.99
CHEMBL485139 1 7.94
CHEMBL483345 1 7.55
CHEMBL484179 1 7.50
CHEMBL482754 1 6.91
CHEMBL482964 1 6.90
CHEMBL485366 1 6.88
CHEMBL485534 1 6.73
CHEMBL482546 1 6.50
CHEMBL520956 1 6.30
CHEMBL519701 1 6.06

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL485356 IC50 = 0.1 nM 10.00
CHEMBL483938 IC50 = 0.31 nM 9.51
CHEMBL521463 IC50 = 0.65 nM 9.19
CHEMBL482764 IC50 = 1.74 nM 8.76
CHEMBL482960 IC50 = 1.89 nM 8.72
CHEMBL484734 IC50 = 2.0 nM 8.70
CHEMBL483344 IC50 = 2.58 nM 8.59
CHEMBL521182 IC50 = 2.95 nM 8.53
CHEMBL485355 IC50 = 3.62 nM 8.44
CHEMBL484733 IC50 = 4.33 nM 8.36
CHEMBL483157 IC50 = 5.43 nM 8.27
CHEMBL520648 IC50 = 5.44 nM 8.26
CHEMBL482974 IC50 = 10.35 nM 7.99
CHEMBL485139 IC50 = 11.55 nM 7.94
CHEMBL483345 IC50 = 28.22 nM 7.55
CHEMBL484179 IC50 = 32.0 nM 7.50
CHEMBL482754 IC50 = 123.6 nM 6.91
CHEMBL482964 IC50 = 124.6 nM 6.90
CHEMBL485366 IC50 = 131.7 nM 6.88
CHEMBL485534 IC50 = 184.9 nM 6.73
CHEMBL482546 IC50 = 314.3 nM 6.50
CHEMBL520956 IC50 = 501.6 nM 6.30
CHEMBL519701 IC50 = 870.8 nM 6.06