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Assay Detail

CHEMBL994325

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of yeast W303 Pho85 phosphorylation assessed as [32P] incorporation in histone H1 from [gamma32P]ATP by scintillation counting
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Saccharomyces cerevisiae
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Cyclin-dependent protein kinase PHO85 (CHEMBL5589)
Type SINGLE PROTEIN
Organism Saccharomyces cerevisiae S288c

Publication

Exploiting chemical libraries, structure, and genomics in the search for kinase inhibitors.
Gray NS, Wodicka L, Thunnissen AM, Norman TC, Kwon S, Espinoza FH, Morgan DO, Barnes G, LeClerc S, Meijer L, Kim SH, Lockhart DJ, Schultz PG.
Science (1998) 281 : 533 -538
PubMed 9677190 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.70 5.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL311228 1 5.70
CHEMBL519036 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL311228 IC50 = 2000.0 nM 5.70
CHEMBL519036 IC50 > 500000.0 nM -