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Assay Detail

CHEMBL997193

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of gamma secretase in human SH-SY5Y cells by HTRF assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SH-SY5Y
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Gamma-secretase (CHEMBL2094135)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Novel orally bioavailable gamma-secretase inhibitors with excellent in vivo activity.
Keown LE, Collins I, Cooper LC, Harrison T, Madin A, Mistry J, Reilly M, Shaimi M, Welch CJ, Clarke EE, Lewis HD, Wrigley JD, Best JD, Murray F, Shearman MS.
J Med Chem (2009) 52 : 3441 -3444
PubMed 19432431 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 9.75 10.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL468083 1 10.52
CHEMBL467457 1 10.22
CHEMBL523832 1 10.22
CHEMBL512282 1 10.22
CHEMBL495009 1 10.22
CHEMBL511572 1 10.15
CHEMBL495185 1 10.10
CHEMBL523883 1 9.92
CHEMBL511928 1 9.82
CHEMBL494588 1 9.77
CHEMBL511375 1 9.68
CHEMBL196215 1 9.62
CHEMBL506323 1 9.47
CHEMBL466421 1 9.39
CHEMBL466256 1 9.38
CHEMBL513674 1 9.38
CHEMBL512596 1 9.34
CHEMBL494769 1 9.09
CHEMBL492394 1 8.78

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL468083 IC50 = 0.03 nM 10.52
CHEMBL467457 IC50 = 0.06 nM 10.22
CHEMBL523832 IC50 = 0.06 nM 10.22
CHEMBL512282 IC50 = 0.06 nM 10.22
CHEMBL495009 IC50 = 0.06 nM 10.22
CHEMBL511572 IC50 = 0.07 nM 10.15
CHEMBL495185 IC50 = 0.08 nM 10.10
CHEMBL523883 IC50 = 0.12 nM 9.92
CHEMBL511928 IC50 = 0.15 nM 9.82
CHEMBL494588 IC50 = 0.17 nM 9.77
CHEMBL511375 IC50 = 0.21 nM 9.68
CHEMBL196215 IC50 = 0.24 nM 9.62
CHEMBL506323 IC50 = 0.34 nM 9.47
CHEMBL466421 IC50 = 0.41 nM 9.39
CHEMBL466256 IC50 = 0.42 nM 9.38
CHEMBL513674 IC50 = 0.42 nM 9.38
CHEMBL512596 IC50 = 0.46 nM 9.34
CHEMBL494769 IC50 = 0.82 nM 9.09
CHEMBL492394 IC50 = 1.65 nM 8.78