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Assay Detail

CHEMBL997215

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human HT-29 cells after 48 hrs by Alamar Blue assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Autocuration

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

Efficient chemoenzymatic synthesis, cytotoxic evaluation, and SAR of epoxysterols.
Carvalho JF, Cruz Silva MM, Moreira JN, Simões S, Sá E Melo ML.
J Med Chem (2009) 52 : 4007 -4019
PubMed 19473028 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 4.74 5.94

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL53463 DOXORUBICIN 4.0 1 5.94
CHEMBL497834 1 5.19
CHEMBL497816 1 5.08
CHEMBL497835 1 4.88
CHEMBL497207 1 4.81
CHEMBL501171 1 4.80
CHEMBL496805 1 4.75
CHEMBL496407 1 4.65
CHEMBL500644 1 4.55
CHEMBL527021 1 4.53
CHEMBL497208 1 4.47
CHEMBL497439 1 4.42
CHEMBL496804 1 4.42
CHEMBL496408 1 4.39
CHEMBL495590 1 4.21
CHEMBL112570 CHOLESTEROL 2.0 1 -
CHEMBL497438 1 -
CHEMBL497411 1 -
CHEMBL498629 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL53463 DOXORUBICIN IC50 = 1150.0 nM 5.94
CHEMBL497834 IC50 = 6400.0 nM 5.19
CHEMBL497816 IC50 = 8300.0 nM 5.08
CHEMBL497835 IC50 = 13300.0 nM 4.88
CHEMBL497207 IC50 = 15500.0 nM 4.81
CHEMBL501171 IC50 = 15900.0 nM 4.80
CHEMBL496805 IC50 = 17700.0 nM 4.75
CHEMBL496407 IC50 = 22600.0 nM 4.65
CHEMBL500644 IC50 = 28200.0 nM 4.55
CHEMBL527021 IC50 = 29400.0 nM 4.53
CHEMBL497208 IC50 = 33800.0 nM 4.47
CHEMBL497439 IC50 = 37600.0 nM 4.42
CHEMBL496804 IC50 = 38200.0 nM 4.42
CHEMBL496408 IC50 = 40900.0 nM 4.39
CHEMBL495590 IC50 = 61900.0 nM 4.21
CHEMBL112570 CHOLESTEROL IC50 > 50000.0 nM -
CHEMBL497438 IC50 > 50000.0 nM -
CHEMBL497411 IC50 > 50000.0 nM -
CHEMBL498629 IC50 > 50000.0 nM -