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Assay Detail

CHEMBL997222

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I][Nle4,Dphe7]-alpha-MSH from human MC1R expressed in HEK293 cells
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocyte-stimulating hormone receptor (CHEMBL3795)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Substitution of arginine with proline and proline derivatives in melanocyte-stimulating hormones leads to selectivity for human melanocortin 4 receptor.
Qu H, Cai M, Mayorov AV, Grieco P, Zingsheim M, Trivedi D, Hruby VJ.
J Med Chem (2009) 52 : 3627 -3635
PubMed 19473029 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 7.69 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL430239 MELATONAN 1 9.70
CHEMBL503229 1 9.30
CHEMBL2096742 1 9.00
CHEMBL524861 1 8.96
CHEMBL502300 1 8.92
CHEMBL501592 1 8.64
CHEMBL500743 1 8.57
CHEMBL526334 1 6.72
CHEMBL508501 1 6.40
CHEMBL194552 1 6.22
CHEMBL510270 1 6.08
CHEMBL525399 1 6.00
CHEMBL455070 1 5.46
CHEMBL509582 1 -
CHEMBL501642 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL430239 MELATONAN IC50 = 0.2 nM 9.70
CHEMBL503229 IC50 = 0.5 nM 9.30
CHEMBL2096742 IC50 = 1.0 nM 9.00
CHEMBL524861 IC50 = 1.1 nM 8.96
CHEMBL502300 IC50 = 1.2 nM 8.92
CHEMBL501592 IC50 = 2.3 nM 8.64
CHEMBL500743 IC50 = 2.7 nM 8.57
CHEMBL526334 IC50 = 190.0 nM 6.72
CHEMBL508501 IC50 = 400.0 nM 6.40
CHEMBL194552 IC50 = 600.0 nM 6.22
CHEMBL510270 IC50 = 830.0 nM 6.08
CHEMBL525399 IC50 = 1000.0 nM 6.00
CHEMBL455070 IC50 = 3500.0 nM 5.46
CHEMBL509582 IC50 > 3000.0 nM -
CHEMBL501642 IC50 > 4800.0 nM -