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Assay Detail

CHEMBL999409

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]flunitrazepam from human recombinant GABAA alpha-1-beta-3-gamma-2 receptor expressed in HEK293 cells
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

GABA-A receptor; alpha-1/beta-3/gamma-2 (CHEMBL2094121)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

A study of the structure-activity relationship of GABA(A)-benzodiazepine receptor bivalent ligands by conformational analysis with low temperature NMR and X-ray analysis.
Han D, Holger Försterling F, Li X, Deschamps JR, Parrish D, Cao H, Rallapalli S, Clayton T, Teng Y, Majumder S, Sankar S, Roth BL, Sieghart W, Furtmuller R, Rowlett JK, Weed MR, Cook JM.
Bioorg Med Chem (2008) 16 : 8853 -8862
PubMed 18790643 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 6.41 7.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL286594 1 7.55
CHEMBL449393 1 6.64
CHEMBL505768 1 6.63
CHEMBL522055 1 6.54
CHEMBL499964 1 6.34
CHEMBL443736 1 5.73
CHEMBL505497 1 5.42
CHEMBL35571 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL286594 Ki = 28.4 nM 7.55
CHEMBL449393 Ki = 231.0 nM 6.64
CHEMBL505768 Ki = 236.0 nM 6.63
CHEMBL522055 Ki = 287.0 nM 6.54
CHEMBL499964 Ki = 460.0 nM 6.34
CHEMBL443736 Ki = 1852.0 nM 5.73
CHEMBL505497 Ki = 3795.0 nM 5.42
CHEMBL35571 Ki > 1000.0 nM -