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Assay Detail

CHEMBL1004121

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant caspase 3 assessed as accumulation of cleaved fluorogenic 7-amino-4-methylcoumarin after 10 mins
21
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Caspase-3 (CHEMBL2334)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Fluorinated isatin derivatives. Part 2. New N-substituted 5-pyrrolidinylsulfonyl isatins as potential tools for molecular imaging of caspases in apoptosis.
Podichetty AK, Wagner S, Schröer S, Faust A, Schäfers M, Schober O, Kopka K, Haufe G.
J Med Chem (2009) 52 : 3484 -3495
PubMed 19445513 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 21 7.49 8.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL293034 1 8.62
CHEMBL493375 1 8.44
CHEMBL523666 1 8.18
CHEMBL513663 1 8.03
CHEMBL467252 1 8.00
CHEMBL511723 1 7.96
CHEMBL62993 1 7.77
CHEMBL513297 1 7.77
CHEMBL524017 1 7.72
CHEMBL521984 1 7.64
CHEMBL512101 1 7.60
CHEMBL468088 1 7.51
CHEMBL494220 1 7.38
CHEMBL467251 1 7.37
CHEMBL513513 1 7.13
CHEMBL492385 1 7.10
CHEMBL60190 1 7.07
CHEMBL466422 1 6.84
CHEMBL493374 1 6.82
CHEMBL466423 1 6.56
CHEMBL466007 1 5.73

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL293034 IC50 = 2.4 nM 8.62
CHEMBL493375 IC50 = 3.6 nM 8.44
CHEMBL523666 IC50 = 6.6 nM 8.18
CHEMBL513663 IC50 = 9.3 nM 8.03
CHEMBL467252 IC50 = 10.0 nM 8.00
CHEMBL511723 IC50 = 11.0 nM 7.96
CHEMBL62993 IC50 = 16.9 nM 7.77
CHEMBL513297 IC50 = 17.0 nM 7.77
CHEMBL524017 IC50 = 19.0 nM 7.72
CHEMBL521984 IC50 = 23.0 nM 7.64
CHEMBL512101 IC50 = 25.0 nM 7.60
CHEMBL468088 IC50 = 31.0 nM 7.51
CHEMBL494220 IC50 = 42.0 nM 7.38
CHEMBL467251 IC50 = 43.0 nM 7.37
CHEMBL513513 IC50 = 74.0 nM 7.13
CHEMBL492385 IC50 = 80.0 nM 7.10
CHEMBL60190 IC50 = 84.9 nM 7.07
CHEMBL466422 IC50 = 145.0 nM 6.84
CHEMBL493374 IC50 = 151.0 nM 6.82
CHEMBL466423 IC50 = 275.0 nM 6.56
CHEMBL466007 IC50 = 1880.0 nM 5.73