Assay Detail
Binding
CHEMBL1006266
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Destabilizing effect on human recombinant PHD2 (181-426) expressed in Escherichia coli BL21 (DE3) assessed as half life by limited trypsinolysis/MALDI-MS analysis in presence of ferrous ion
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 0 — Uncurated / Unknown |
| Curated By | Autocuration |
Publication
Application of a proteolysis/mass spectrometry method for investigating the effects of inhibitors on hydroxylase structure.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 7 | 5.45 | 7.14 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL426560 | — | — | 1 | 7.14 |
| CHEMBL485022 | — | — | 1 | 6.29 |
| CHEMBL316034 | — | — | 1 | 5.72 |
| CHEMBL90852 | N-OXALYLGLYCINE | — | 1 | 4.73 |
| CHEMBL503160 | FUMARIC ACID | 3.0 | 1 | 4.72 |
| CHEMBL576 | SUCCINIC ACID | 3.0 | 1 | 4.07 |
| CHEMBL485023 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL426560 | — | IC50 | = | 73.0 | nM | 7.14 |
| CHEMBL485022 | — | IC50 | = | 512.0 | nM | 6.29 |
| CHEMBL316034 | — | IC50 | = | 1910.0 | nM | 5.72 |
| CHEMBL90852 | N-OXALYLGLYCINE | IC50 | = | 18500.0 | nM | 4.73 |
| CHEMBL503160 | FUMARIC ACID | IC50 | = | 19090.0 | nM | 4.72 |
| CHEMBL576 | SUCCINIC ACID | IC50 | = | 85300.0 | nM | 4.07 |
| CHEMBL485023 | — | IC50 | = | 230000.0 | nM | - |