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Assay Detail

CHEMBL1009321

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against human BT474 cells overexpressing ERBb2 after 3 days by methylene blue staining
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type BT-474
Confidence 1 — Organism
Curated By Autocuration

Target

BT-474 (CHEMBL614529)
Type CELL-LINE
Organism Homo sapiens

Publication

Synthesis and stereochemical effects of pyrrolidinyl-acetylenic thieno[3,2-d]pyrimidines as EGFR and ErbB-2 inhibitors.
Stevens KL, Alligood KJ, Alberti JG, Caferro TR, Chamberlain SD, Dickerson SH, Dickson HD, Emerson HK, Griffin RJ, Hubbard RD, Keith BR, Mullin RJ, Petrov KG, Gerding RM, Reno MJ, Rheault TR, Rusnak DW, Sammond DM, Smith SC, Uehling DE, Waterson AG, Wood ER.
Bioorg Med Chem Lett (2009) 19 : 21 -26
PubMed 19028424 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 22 7.27 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL466175 1 8.00
CHEMBL31965 CANERTINIB 3.0 1 8.00
CHEMBL1201179 LAPATINIB DITOSYLATE 4.0 1 7.70
CHEMBL466174 1 7.70
CHEMBL461426 1 7.60
CHEMBL466412 GSK-238063A 1 7.52
CHEMBL507329 GW869810X 1 7.52
CHEMBL517123 GSK-182497A 1 7.52
CHEMBL512076 GSK-192082A 1 7.52
CHEMBL466586 1 7.52
CHEMBL450109 1 7.30
CHEMBL451513 1 7.22
CHEMBL461869 1 7.22
CHEMBL505626 1 7.10
CHEMBL517585 1 7.05
CHEMBL499534 1 7.00
CHEMBL450396 1 6.92
CHEMBL466614 1 6.89
CHEMBL447365 1 6.85
CHEMBL466615 1 6.64
CHEMBL504034 1 6.54
CHEMBL939 GEFITINIB 4.0 1 6.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL466175 EC50 = 10.0 nM 8.00
CHEMBL31965 CANERTINIB EC50 = 10.0 nM 8.00
CHEMBL1201179 LAPATINIB DITOSYLATE EC50 = 20.0 nM 7.70
CHEMBL466174 EC50 = 20.0 nM 7.70
CHEMBL461426 EC50 = 25.0 nM 7.60
CHEMBL466412 GSK-238063A EC50 = 30.0 nM 7.52
CHEMBL507329 GW869810X EC50 = 30.0 nM 7.52
CHEMBL517123 GSK-182497A EC50 = 30.0 nM 7.52
CHEMBL512076 GSK-192082A EC50 = 30.0 nM 7.52
CHEMBL466586 EC50 = 30.0 nM 7.52
CHEMBL450109 EC50 = 50.0 nM 7.30
CHEMBL451513 EC50 = 60.0 nM 7.22
CHEMBL461869 EC50 = 60.0 nM 7.22
CHEMBL505626 EC50 = 80.0 nM 7.10
CHEMBL517585 EC50 = 90.0 nM 7.05
CHEMBL499534 EC50 = 100.0 nM 7.00
CHEMBL450396 EC50 = 120.0 nM 6.92
CHEMBL466614 EC50 = 130.0 nM 6.89
CHEMBL447365 EC50 = 140.0 nM 6.85
CHEMBL466615 EC50 = 230.0 nM 6.64
CHEMBL504034 EC50 = 290.0 nM 6.54
CHEMBL939 GEFITINIB EC50 = 300.0 nM 6.52