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Assay Detail

CHEMBL1009645

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human recombinant EP1 receptor expressed in CHO cells assessed as inhibition of PGE2-mediated intracellular calcium mobilization by FLIPR method
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin E2 receptor EP1 subtype (CHEMBL1811)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of sodium 6-[(5-chloro-2-{[(4-chloro-2-fluorophenyl)methyl]oxy}phenyl)methyl]-2-pyridinecarboxylate (GSK269984A) an EP(1) receptor antagonist for the treatment of inflammatory pain.
Hall A, Billinton A, Brown SH, Chowdhury A, Clayton NM, Giblin GM, Gibson M, Goldsmith PA, Hurst DN, Naylor A, Peet CF, Scoccitti T, Wilson AW, Winchester W.
Bioorg Med Chem Lett (2009) 19 : 2599 -2603
PubMed 19332369 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 7.57 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL467114 1 8.10
CHEMBL467720 1 7.60
CHEMBL513491 1 7.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL467114 Ki = 7.943 nM 8.10
CHEMBL467720 Ki = 25.12 nM 7.60
CHEMBL513491 Ki = 100.0 nM 7.00