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Assay Detail

CHEMBL1009646

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]PGE2 from human EP1 receptor expressed in CHO cells
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin E2 receptor EP1 subtype (CHEMBL1811)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of sodium 6-[(5-chloro-2-{[(4-chloro-2-fluorophenyl)methyl]oxy}phenyl)methyl]-2-pyridinecarboxylate (GSK269984A) an EP(1) receptor antagonist for the treatment of inflammatory pain.
Hall A, Billinton A, Brown SH, Chowdhury A, Clayton NM, Giblin GM, Gibson M, Goldsmith PA, Hurst DN, Naylor A, Peet CF, Scoccitti T, Wilson AW, Winchester W.
Bioorg Med Chem Lett (2009) 19 : 2599 -2603
PubMed 19332369 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.62 9.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2110364 1 9.89
CHEMBL234940 1 8.60
CHEMBL233043 1 8.00
CHEMBL467720 1 8.00
CHEMBL513491 1 7.90
CHEMBL467114 1 7.90
CHEMBL467510 1 7.80
CHEMBL466101 1 7.60
CHEMBL511252 1 7.50
CHEMBL466103 1 7.40
CHEMBL467113 1 7.40
CHEMBL257998 1 7.40
CHEMBL466102 1 7.10
CHEMBL508276 1 7.10
CHEMBL511733 1 7.00
CHEMBL468723 1 7.00
CHEMBL258041 1 6.80
CHEMBL511919 1 6.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2110364 IC50 = 0.1288 nM 9.89
CHEMBL234940 IC50 = 2.512 nM 8.60
CHEMBL233043 IC50 = 10.0 nM 8.00
CHEMBL467720 IC50 = 10.0 nM 8.00
CHEMBL513491 IC50 = 12.59 nM 7.90
CHEMBL467114 IC50 = 12.59 nM 7.90
CHEMBL467510 IC50 = 15.85 nM 7.80
CHEMBL466101 IC50 = 25.12 nM 7.60
CHEMBL511252 IC50 = 31.62 nM 7.50
CHEMBL466103 IC50 = 39.81 nM 7.40
CHEMBL467113 IC50 = 39.81 nM 7.40
CHEMBL257998 IC50 = 39.81 nM 7.40
CHEMBL466102 IC50 = 79.43 nM 7.10
CHEMBL508276 IC50 = 79.43 nM 7.10
CHEMBL511733 IC50 = 100.0 nM 7.00
CHEMBL468723 IC50 = 100.0 nM 7.00
CHEMBL258041 IC50 = 158.49 nM 6.80
CHEMBL511919 IC50 = 199.53 nM 6.70