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Assay Detail

CHEMBL1010964

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at rat recombinant GnRH receptor assessed as inhibition of [D-Trp6]-GnRH-stimulated LH release from rat pituitary cells
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Cell Type Pituitary gland cell
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Gonadotropin-releasing hormone receptor (CHEMBL3066)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Discovery of 6-({4-[2-(4-tert-butylphenyl)-1H-benzimidazol-4-yl]piperazin-1-yl}methyl)quinoxaline (WAY-207024): an orally active antagonist of the gonadotropin releasing hormone receptor (GnRH-R).
Pelletier JC, Chengalvala MV, Cottom JE, Feingold IB, Green DM, Hauze DB, Huselton CA, Jetter JW, Kopf GS, Lundquist JT, Magolda RL, Mann CW, Mehlmann JF, Rogers JF, Shanno LK, Adams WR, Tio CO, Wrobel JE.
J Med Chem (2009) 52 : 2148 -2152
PubMed 19271735 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.64 7.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL459215 1 7.60
CHEMBL513998 1 7.36
CHEMBL446978 1 7.16
CHEMBL475650 1 6.92
CHEMBL473807 1 6.62
CHEMBL474991 1 6.50
CHEMBL475660 1 6.27
CHEMBL474992 1 6.08
CHEMBL515123 1 5.23

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL459215 IC50 = 25.0 nM 7.60
CHEMBL513998 IC50 = 44.0 nM 7.36
CHEMBL446978 IC50 = 69.0 nM 7.16
CHEMBL475650 IC50 = 120.0 nM 6.92
CHEMBL473807 IC50 = 240.0 nM 6.62
CHEMBL474991 IC50 = 320.0 nM 6.50
CHEMBL475660 IC50 = 540.0 nM 6.27
CHEMBL474992 IC50 = 830.0 nM 6.08
CHEMBL515123 IC50 = 5900.0 nM 5.23