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Assay Detail

CHEMBL1011629

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of TNFalpha production in LPS-stimulated human whole blood preincubated before LPS challenge measured after 4 hrs by enzyme immunoassay
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Tissue Blood
Confidence 1 — Organism
Curated By Autocuration

Target

Blood (CHEMBL613416)
Type TISSUE
Organism Homo sapiens

Publication

Discovery of (S)-N-[2-[1-(3-ethoxy-4-methoxyphenyl)-2-methanesulfonylethyl]-1,3-dioxo-2,3-dihydro-1H-isoindol-4-yl] acetamide (apremilast), a potent and orally active phosphodiesterase 4 and tumor necrosis factor-alpha inhibitor.
Man HW, Schafer P, Wong LM, Patterson RT, Corral LG, Raymon H, Blease K, Leisten J, Shirley MA, Tang Y, Babusis DM, Chen R, Stirling D, Muller GW.
J Med Chem (2009) 52 : 1522 -1524
PubMed 19256507 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 5.85 6.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL514800 APREMILAST 4.0 1 6.96
CHEMBL475165 1 6.36
CHEMBL313439 1 6.31
CHEMBL474557 1 5.85
CHEMBL472963 1 5.80
CHEMBL474556 1 5.66
CHEMBL473205 1 5.62
CHEMBL514339 1 5.60
CHEMBL88475 1 5.40
CHEMBL419135 1 4.98
CHEMBL473983 1 -
CHEMBL516063 1 -
CHEMBL515728 1 -
CHEMBL508838 1 -
CHEMBL515398 1 -
CHEMBL473204 1 -
CHEMBL90345 1 -
CHEMBL475164 1 -
CHEMBL315529 1 -
CHEMBL88153 CDC-801 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL514800 APREMILAST IC50 = 110.0 nM 6.96
CHEMBL475165 IC50 = 440.0 nM 6.36
CHEMBL313439 IC50 = 490.0 nM 6.31
CHEMBL474557 IC50 = 1400.0 nM 5.85
CHEMBL472963 IC50 = 1600.0 nM 5.80
CHEMBL474556 IC50 = 2200.0 nM 5.66
CHEMBL473205 IC50 = 2400.0 nM 5.62
CHEMBL514339 IC50 = 2500.0 nM 5.60
CHEMBL88475 IC50 = 4000.0 nM 5.40
CHEMBL419135 IC50 = 10500.0 nM 4.98
CHEMBL473983 IC50 - -
CHEMBL516063 IC50 - -
CHEMBL515728 IC50 - -
CHEMBL508838 IC50 - -
CHEMBL515398 IC50 - -
CHEMBL473204 IC50 - -
CHEMBL90345 IC50 - -
CHEMBL475164 IC50 - -
CHEMBL315529 IC50 - -
CHEMBL88153 CDC-801 IC50 - -